For the effective wound management Plasmin can be combined with Deoxyribonuclease (DNase). The mixture of these two enzymes is called Bionuclease.
Mechanism of action on wounds
The two enzymes in Bionuclease work simultaneously to enhance wound cleaning and accelerate the healing process. Plasmin acts as a wound debridement agent; it attacks and dissolves clots of fibrin. In other words, plasmin clears up a wound removing dead skin and tissue. Deoxyribonuclease, on the other hand, attacks DNA found in purulent exudates.
Application in ointments
Ointments with Bionuclease are indicated for promoting healing of wounds, in particular chronic wounds and wounds with pre-existing physiological condition, e.g. diabetic foot ulcers, general surgical wounds, ulcerative lesions-trophic, abscesses, decubitus, stasis, fistulas, circumcision and episiotomy. The ointments are administrated intravaginally in conditions as cervicitis-benign, postpartum and postconization vaginitis.
BIOFAC Plasmin for induction of PVD
Plasmin products have shown to be effective for the induction of posterior vitreous detachment (PVD), a technique which is considered beneficial in patients with numerous retinal conditions, including diabetic retinopathy which is damage to the small blood vessels in the retina that can result in loss of vision.
BIOFAC Plasmin grades
BIOFAC offers pure Plasmin as well as its mixture with Deoxyribonuclease as follows:
- Pure BIOFAC Plasmin grade with activity of 0.3 – 0.5 LU/mg.
- Bionuclease:.a mixture of two enzymes: BIOFAC Plasmin and Deoxyribonuclease (DNase) in ratio 1:666
- Bionuclease: a mixture of BIOFAC Plasmin and Deoxyribonuclease (DNase)in other ratios, e.g. 1:800 or 1:1200.
Storage and Stability
BIOFAC Plasmin is a lyophilized powder that easily absorbs humidity from the air. It should be stored at 2-8°C in tight containers. The re-test date for BIOFAC Plasmin is 3 years from the date of production.
A multiple role of Plasmin during wound healing
Plasmin is believed to play a multiple role during wound healing. In particular, plasmin degrades an extracellular matrix.
What is more, plasmin promotes cell activation and expression of the pro- inflammatory mediators, which are crucial for wound healing. Finally, In vitro studies demonstrate that Plasmin induces signaling pathways promoting wound healing.
The serine protease plasmin triggers expression of MCP-1 and CD40 in human primary monocytes via activation of p38 MAPK and janus kinase (JAK)/STAT signalling pathways (Burysek, L et al.; J Biol Chem. 2002)
Molecular mechanisms of fibrinolysis. (Cesarman-Maus G Hajjar KA.; Br J Haematol. 2005)
Plasmin in healing of diabetic wounds
Falanga V. Wound healing and its impairment in the diabetic foot. Lancet. 005;366(9498):1736-1743.Shen Y, Guo Y, Mikus P, Sulniute R, Wilczynska M, Ny T, and Li J.
Plasminogen is a key pro-inflammatory regulator that accelerates the healing of acute and diabetic wounds. Blood. 119:5879-5887, 2012.
Posterior vitreous detachment with plasmin in the isolated human eye. Li, X et al.; Graefes Arch Clin Exp Ophthalmol. 2002